Employing chromatographic and spectroscopic methodologies to monitor DNA response to platinum-based chemotherapeutic drugs

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dc.contributor.advisorMirsaleh-Kohan, Nasrin
dc.creatorWappes, Skylar C
dc.creator.orcid0000-0003-0644-3438
dc.date.accessioned2020-08-20T19:17:36Z
dc.date.created2020-05
dc.date.issued6/16/2020
dc.date.submitted20-May
dc.date.updated2020-08-20T19:17:37Z
dc.description.abstractIn order to design platinum-based chemotherapeutic drugs that express low toxicity and high efficiency, their nature of interaction with DNA, effectiveness, as well as their mechanisms need to be better understood. This research investigates how Cisplatin, cis-diamminedichloroplatinum (II) and its derivatives, Carboplatin, Nedaplatin, and Oxaliplatin, effect DNA stability and structure. Two sensitive and selective analytical methodologies - Surface-Enhanced Raman Scattering (SERS) and High-Performance Liquid Chromatography (HPLC)- were applied to monitor the key concepts stated above. In this project, it was found that of all the drugs, Carboplatin did not significantly modify the DNA. Even though Carboplatin complexes were made at a 1:2 volume ratio it left more DNA unbound (5% unbound) compared to the other drug complexes which were ran at a 1:1 ratio (<2% unbound). Averaging the drug interactions, Cisplatin and Oxaliplatin left less DNA unbound than Nedaplatin did, but Carboplatin in all HPLC experiments left significantly more DNA unbound. In both SERS and HPLC experiments, Carboplatin modified each DNA less than Cisplatin.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/11274/12433
dc.language.isoen
dc.subjectCisplatin
dc.subjectCarboplatin
dc.subjectNedaplatin
dc.subjectOxaliplatin
dc.subjectDNA
dc.subjectHPLC
dc.subjectSERS
dc.titleEmploying chromatographic and spectroscopic methodologies to monitor DNA response to platinum-based chemotherapeutic drugs
dc.typeThesis
dc.type.materialtext
local.embargo.lift5/1/2023
local.embargo.terms5/1/2023
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineChemistry
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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Chemistry & Biochemistry
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