Characterizing the thermodynamic parameters of anti-cancer drug Carboplatin and DNA through Spectroscopic techniques

Williams, Sara M
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Spectroscopic techniques have been employed to understand the interactions and thermodynamic parameters between the anti-cancer drug Carboplatin and the DNA oligomer COTAR 2: ATT AAT GGA TCC ATT AAT. This is a self-complementary sequence that has been previously shown to bind [Co(NH3)2(OH2)2]+3 with high specificity and is of interest because it contains two isolated G-G sites. Both Cisplatin and Carboplatin are known for binding preferentially to G-G sites. These anti-cancer drugs are commonly used in chemotherapeutic treatments and are known to have adverse side effects. In order to improve treatment options, it’s necessary to understand the molecular basis of their interactions with DNA. The binding of Carboplatin with COTAR 2 has been analyzed using Circular Dichroism (CD), Surface−Enhanced Raman Scattering (SERS), and UV-Vis Spectroscopy. The combination of these techniques allows for better understanding of structure and stability of platinum-DNA complexes, as well as binding kinetics. In this thesis, the results of these studies will be presented, and our current understanding of this interaction will be discussed.

Spectroscopy, Surface Enhanced Raman Scattering, Nanoparticles, Cisplatin, Carboplatin, UV-Visible Spectroscopy, Circular Dichroism