Characterizing the thermodynamic parameters of anti-cancer drug Carboplatin and DNA through Spectroscopic techniques

dc.contributor.advisorMirsaleh-Kohan, Nasrin
dc.creatorWilliams, Sara M
dc.creator.orcid0000-0003-1209-8941
dc.date.accessioned2020-08-20T19:27:42Z
dc.date.available2020-08-20T19:27:42Z
dc.date.created2020-05
dc.date.issued6/3/2020
dc.date.submitted20-May
dc.date.updated2020-08-20T19:27:42Z
dc.description.abstractSpectroscopic techniques have been employed to understand the interactions and thermodynamic parameters between the anti-cancer drug Carboplatin and the DNA oligomer COTAR 2: ATT AAT GGA TCC ATT AAT. This is a self-complementary sequence that has been previously shown to bind [Co(NH3)2(OH2)2]+3 with high specificity and is of interest because it contains two isolated G-G sites. Both Cisplatin and Carboplatin are known for binding preferentially to G-G sites. These anti-cancer drugs are commonly used in chemotherapeutic treatments and are known to have adverse side effects. In order to improve treatment options, it’s necessary to understand the molecular basis of their interactions with DNA. The binding of Carboplatin with COTAR 2 has been analyzed using Circular Dichroism (CD), Surface−Enhanced Raman Scattering (SERS), and UV-Vis Spectroscopy. The combination of these techniques allows for better understanding of structure and stability of platinum-DNA complexes, as well as binding kinetics. In this thesis, the results of these studies will be presented, and our current understanding of this interaction will be discussed.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/11274/12441
dc.language.isoen
dc.subjectSpectroscopy
dc.subjectSurface Enhanced Raman Scattering
dc.subjectNanoparticles
dc.subjectCisplatin
dc.subjectCarboplatin
dc.subjectUV-Visible Spectroscopy
dc.subjectCircular Dichroism
dc.titleCharacterizing the thermodynamic parameters of anti-cancer drug Carboplatin and DNA through Spectroscopic techniques
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineChemistry
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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