Nutrition & Food Sciences

Permanent URI for this collectionhttps://hdl.handle.net/11274/8856

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Browsing Nutrition & Food Sciences by Subject "Adipocyte differentiation"

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    Effect of blueberry polyphenols on 3T3-F442A preadipocyte differentiation
    (2010-12) Moghe, Shiwani; Vijayagopal, Parakat; Irmhan, Victorine; Juma, Shanil
    Today obesity is an epidemic and its prevalence has increased significantly over the last few decades. To avoid excessive accumulation of fat, optimum energy intake along with regular exercise is mandatory. Polyphenols present in green tea, grape seeds, orange and grapefruit combat adipogenesis at the molecular level and also induce lipolysis. However, very little is known regarding the role of blueberry polyphenols on adipocyte differentiation. Hence we tested the dose-dependent effects of blueberry polyphenols on mouse 3T3-F442A preadipocyte differentiation and lipolysis. 3T3-F442A preadipocytes were incubated with three doses of blueberry polyphenols (BB-150, BB-200 and BB-250 μg/mL) and intracellular lipid content, cell proliferation and lipolysis were assayed. Blueberry polyphenols showed a dose-dependent suppression of adipocyte differentiation determined by oil red-O staining and adipored assay. Intracellular lipid content in control (11,385.51 ± 1169.6, relative fluorescence units) was significantly higher (P < 0.05) than the three doses of blueberry polyphenols (8336.86 ± 503.57), (4235.67 ± 323.17), and (3027.97 ± 346.61). This corresponds to a reduction of 27%, 63% and 74%, respectively. Cell proliferation was observed to be significantly high in control (0.744 ± 0.035, optical density units) than BB-150 (0.517 ± 0.031), BB-200 (0.491 ± 0.023), and BB-250 (0.455 ± 0.012). However, when tested for lipolysis, there was no significant difference observed among the groups. We conclude that blueberry polyphenols may play an effective role in inhibiting adipogenesis and cell proliferation. Keywords: Obesity; adipocyte differentiation; polyphenols; Blueberry; cell proliferation; lipolysis
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    Effects of curcumin on retinoblastoma protein and p300 during adipocyte differentiation
    (1/9/2020) Rea, Jonathan A.; Vijayagopal, Parakat; Imrhan, Victorine; Prasad, Chandan
    The goal of the present study was to understand how curcumin suppresses PPARγ-mediated adipocyte differentiation in 3T3-F442 preadipocytes. We differentiated cultured 3T3-F442 cells with and without different doses of curcumin (0, 10µM, 15µM, and 20µM) and prepared cell lysates. We then performed Western Blot analysis to measure two upstream regulators of PPARγ, retinoblastoma protein (RB) and the p300 histone acetyltransferase in preadipocyte cells. Results were analyzed using one-Way ANOVA, and a Tuckey Post Hoc test. Curcumin caused a dose-dependent inhibition of adipocyte differentiation. Compared to control (no curcumin), dephosphorylated RB protein expression was higher in cultures exposed to 10 µM and 15 µM curcumin. However, at 20 µM the unphosphorylated RB protein decreased to the control level probably due to cell death. This suggests that curcumin inhibits 3T3-F442 preadipocyte differentiation by RB protein mediated inhibition of PPAR- γ. We did not detect the P300 protein in any cultures, including control by Western blot. This may be due to several reasons, including the cells not expressing P300, low sensitivity of detection technique, or denaturation of protein.