Effects of curcumin on retinoblastoma protein and p300 during adipocyte differentiation

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dc.contributor.committeeMemberVijayagopal, Parakat
dc.contributor.committeeMemberImrhan, Victorine
dc.contributor.committeeMemberPrasad, Chandan
dc.creatorRea, Jonathan A.
dc.creator.orcid0000-0002-4700-5007
dc.date.accessioned2020-01-30T15:03:28Z
dc.date.available2020-01-30T15:03:28Z
dc.date.created2019-12
dc.date.issued1/9/2020
dc.date.submitted19-Dec
dc.date.updated2020-01-30T15:03:28Z
dc.description.abstractThe goal of the present study was to understand how curcumin suppresses PPARγ-mediated adipocyte differentiation in 3T3-F442 preadipocytes. We differentiated cultured 3T3-F442 cells with and without different doses of curcumin (0, 10µM, 15µM, and 20µM) and prepared cell lysates. We then performed Western Blot analysis to measure two upstream regulators of PPARγ, retinoblastoma protein (RB) and the p300 histone acetyltransferase in preadipocyte cells. Results were analyzed using one-Way ANOVA, and a Tuckey Post Hoc test. Curcumin caused a dose-dependent inhibition of adipocyte differentiation. Compared to control (no curcumin), dephosphorylated RB protein expression was higher in cultures exposed to 10 µM and 15 µM curcumin. However, at 20 µM the unphosphorylated RB protein decreased to the control level probably due to cell death. This suggests that curcumin inhibits 3T3-F442 preadipocyte differentiation by RB protein mediated inhibition of PPAR- γ. We did not detect the P300 protein in any cultures, including control by Western blot. This may be due to several reasons, including the cells not expressing P300, low sensitivity of detection technique, or denaturation of protein.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/11274/12122
dc.language.isoen
dc.subject3T3-F442A Cells
dc.subjectAdipocyte differentiation
dc.subjectp300 histone acetyltransferase
dc.subjectRetinoblastoma protein
dc.titleEffects of curcumin on retinoblastoma protein and p300 during adipocyte differentiation
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentNutrition and Food Sciences
thesis.degree.disciplineNutrition
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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