Quantitative age grading of mosquitoes using surface‐enhanced Raman spectroscopy

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dc.contributor.authorWang, Danhui
dc.contributor.authorYang, Jason
dc.contributor.authorPandya, Janam
dc.contributor.authorClark, John M.
dc.contributor.authorHarrington, Laura C.
dc.contributor.authorMurdock, Courtney C.
dc.contributor.authorHe, Lili
dc.date.accessioned2023-03-22T17:56:22Z
dc.date.available2023-03-22T17:56:22Z
dc.date.issued2021
dc.descriptionArticle originally published in Analytical Science Advances, 3(1-2), 47–53. https://doi.org/10.1002/ansa.202100052
dc.description.abstractMosquito-borne pathogens, including malaria, Zika, dengue, and chikungunya continue to be a major public health concern globally. Based on the understanding that only older female mosquitoes are infectious and represent a risk to human health, scientists have sought to age-grade mosquitoes for decades. To date, however, no reliable, cost-effective and practical methods exist to age older mosquitoes despite the tremendous epidemiological value of this approach. This study is the first attempt to develop a surface-enhanced Raman spectroscopic (SERS) method to age mosquitoes. The water extracts of Aedes aegypti mosquitoes aged 0–22 days were mixed with silver nanoparticles. The SERS spectra, which were analysed by principal component analysis and partial least square (PLS), demonstrated the capability of this approach to predict the calendar age of mosquitoes between 0 and 22 days with the coefficient of correlation (R) = 0.994 and 0.990 for PLS model calibration and validation, respectively. Spectral analysis with both SERS and infrared spectroscopy revealed the key biological sources leading to changes in spectra allowing mosquito age-grading is adenine-containing compounds and proteins. In addition, we evaluated the impact of two arthropod-borne pathogen deactivating pre-treatments (bleach and ethanol) on the discrimination capability of the SERS approach. The result shows the ethanol treatment has the potential to enhance the discrimination capability and the safety of the approach. This study represents the first step towards developing the SERS approach as a quick, reliable and field-deployable method for mosquito age-grading, which would significantly improve the effectiveness of vector-borne disease monitoring and prevention.en_US
dc.identifier.citationThis is a published version of an article that is available at https://doi.org/10.1002/ansa.202100052. Recommended citation: Wang, D., Yang, J., Pandya, J., Clark, J. M., Harrington, L. C., Murdock, C. C., & He, L. (2021). Quantitative age grading of mosquitoes using surface‐enhanced Raman spectroscopy. Analytical Science Advances, 3(1-2), 47–53. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.en_US
dc.identifier.urihttps://hdl.handle.net/11274/14710
dc.identifier.urihttps://doi.org/10.1002/ansa.202100052
dc.language.isoen_USen_US
dc.publisherWiley Open Accessen_US
dc.rights.licenseCC BY
dc.subjectAgeen_US
dc.subjectMosquitoesen_US
dc.subjectSERSen_US
dc.subjectQuantitativeen_US
dc.titleQuantitative age grading of mosquitoes using surface‐enhanced Raman spectroscopyen_US
dc.typeArticleen_US

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