Understanding the conformations and stabilities of g-quadruplexes formed by human telomere sequence in ideal and less ideal solutions

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dc.contributor.authorSharma, Vishal Rajaten_US
dc.contributor.committeeChairSheardy, Richard Dean
dc.contributor.committeeMemberMirsaleh-Kohan, Nasrin
dc.contributor.committeeMemberRawashdeh-Omary, Manal
dc.date.accessioned2014-12-10T19:19:03Z
dc.date.available2014-12-10T19:19:03Z
dc.date.copyright2014en_US
dc.date.issued1/1/2014en_US
dc.description.abstractThe G-rich termini of human chromosomes, called telomeres, have the ability to form unique DNA structures called G-Quadruplexes, which have been implicated in certain cancer types and, as a result, have drawn researchers' attention to study their structure inside the cell and thus consider them as potential targets for new anticancer therapies. Biophysical studies of these entities have been carried out under near ideal conditions, i.e., low phosphate concentration (e.g., 10mM), pH 7, low ionic strength (e.g., 115mM), and for optical studies, low concentration of DNA (e.g., 1 × 10-6 M). We, here, created less ideal conditions by using different osmolytes such as polyethylene glycol, acetonitrile, trifluoroethanol, and betaine. Our research focuses on the effect of these co-solutes on the structural and thermodynamic properties of telomeric DNA oligomers and report the characterization of (TTAGGG)x, where x = 1, 2, or 4. In general, increasing the percentage of any osmolytes in solutions drives the equilibrium from unimolecular hybrid to multimolecular parallel conformation. In addition, Stability of parallel conformation also increases.en_US
dc.identifier.urihttp://hdl.handle.net/11274/3680
dc.language.isoen_USen_US
dc.subjectMolecular biology
dc.subjectBiochemistry
dc.subjectBiophysics
dc.subjectPure sciences
dc.subjectChemistryen_US
dc.titleUnderstanding the conformations and stabilities of g-quadruplexes formed by human telomere sequence in ideal and less ideal solutionsen_US
dc.typeThesisen_US
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineChemistry
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMaster
thesis.degree.nameMaster of Chemistry

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