Global chromatin compaction in response to ultraviolet radiation

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dc.contributor.authorSinha Roy, Rituparna
dc.contributor.authorKenning, Abigail
dc.contributor.authorAbbas, Mohammad
dc.contributor.authorBergel, Michael
dc.date.accessioned2021-03-19T23:57:10Z
dc.date.available2021-03-19T23:57:10Z
dc.date.issued2021
dc.descriptionCreative Arts and Research Symposium
dc.descriptionCreative Arts and Research Symposiumen_US
dc.description.abstractChromatin, the complex of DNA, associated proteins, and RNA has several levels of folding. Gene expression, DNA replication, and DNA repair are cellular functions dependent on unfolding compacted chromatin. This study aims to understand the relationship between UV radiation and the compaction level of chromatin within a cell. Large scale chromatin compaction after UV radiation was observed by a hybrid protein LacR-CFP tethered to a 256 tandemly repeated LacO DNA sequences integrated into the genome of NIH2/4 mouse embryonic fibroblasts. We further corroborated global chromatin compaction in human cervical cancer HeLa cells and in normal human epidermal melanocytes irradiated by UV-B, using two fluorescent dyes and we demonstrated that this compaction protects the DNA from further damage using immunostaining. We also have shown that UV-induced chromatin compaction is dependent on the influx of calcium ions into the nucleus. We currently plan to explore the pathway controlling the UV-induced calcium influx.
dc.description.departmentBiology
dc.description.sponsorshipSupported by an NIH R03 grant, TWU Center for Student Research and TWU Pioneer Center for Student Excellence
dc.identifier.urihttps://hdl.handle.net/11274/12911
dc.language.isoen_USen_US
dc.titleGlobal chromatin compaction in response to ultraviolet radiationen_US
dc.typePosteren_US

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