The elongin B ubiquitin homology domain




Brower, Christopher S.
Shilatifard, Ali
Mather, Timothy
Kamura, Takumi
Takagi, Yuichiro
Haque, Dewan
Treharne, Annemarie
Foundling, Stephen I.
Conaway, Joan Weliky
Conaway, Ronald C.

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Mammalian Elongin B is a 118-amino acid protein composed of an 84-amino acid amino-terminal ubiquitin-like domain and a 34-amino acid carboxyl-terminal tail. Elongin B is found in cells as a subunit of the heterodimeric Elongin BC complex, which was originally identified as a positive regulator of RNA polymerase II elongation factor Elongin A and subsequently as a component of the multiprotein von Hippel-Lindau tumor suppressor and suppressor of cytokine signaling complexes. As part of our effort to understand how the Elongin BC complex regulates the activity of Elongin A, we are characterizing Elongin B functional domains. In this report, we show that the Elongin B ubiquitin-like domain is necessary and sufficient for interaction with Elongin C and for positive regulation of Elongin A transcriptional activity. In addition, by site-directed mutagenesis of the Elongin B ubiquitin-like domain, we identify a short Elongin B region that is important for its interaction with Elongin C. Finally, we observe that both the ubiquitin-like domain and carboxyl-terminal tail are conserved in Drosophila melanogaster and Caenorhabditis elegans Elongin B homologs that efficiently substitute for mammalian Elongin B in reconstitution of the transcriptionally active Elongin ABC complex, suggesting that the carboxyl-terminal tail performs an additional function not detected in our assays.


Article originally published by Journal of Biological Chemistry, 274(19). English. Published online 2002.


Multiprotein von Hippel-Lindau tumor suppressor, Cytokine signaling complexes, Site-directed mutagenesis


This is the published version of an article that is available at Recommended citation: Brower, C. S., Shilatifard, A., Mather, T., Kamura, T., Takagi, Y., Haque, D., Treharne, A., Foundling, S. I., Conaway, J. W., & Conaway, R. C. (1999). The elongin B ubiquitin homology domain. Journal of Biological Chemistry, 274(19), 13629–13636. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.