Genetic interactions between the DBL-1/BMP-like pathway and dpy body size–associated genes in Caenorhabditis elegans

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dc.contributor.authorLakdawala, Mohammed Farhan
dc.contributor.authorMadhu, Bhoomi
dc.contributor.authorFaure, Lionel
dc.contributor.authorVora, Mehul
dc.contributor.authorPadgett, Richard W.
dc.contributor.authorGumienny, Tina L.
dc.creator.orcidhttps://orcid.org/0000-0002-3932-7815
dc.date.accessioned2021-02-01T19:13:15Z
dc.date.available2021-02-01T19:13:15Z
dc.date.issued2019-12-12
dc.description.abstractBone morphogenetic protein (BMP) signaling pathways control many developmental and homeostatic processes, including cell size and extracellular matrix remodeling. An understanding of how this pathway itself is controlled remains incomplete. To identify novel regulators of BMP signaling, we performed a forward genetic screen in Caenorhabditis elegans for genes involved in body size regulation, a trait under the control of BMP member DBL-1. We isolated mutations that suppress the long phenotype of lon-2, a gene that encodes a negative regulator that sequesters DBL-1. This screen was effective because we isolated alleles of several core components of the DBL-1 pathway, demonstrating the efficacy of the screen. We found additional alleles of previously identified but uncloned body size genes. Our screen also identified widespread involvement of extracellular matrix proteins in DBL-1 regulation of body size. We characterized interactions between the DBL-1 pathway and extracellular matrix and other genes that affect body morphology. We discovered that loss of some of these genes affects the DBL-1 pathway, and we provide evidence that DBL-1 signaling affects many molecular and cellular processes associated with body size. We propose a model in which multiple body size factors are controlled by signaling through the DBL-1 pathway and by DBL-1–independent processes.en_US
dc.identifier.citationThis is the published version of an article that is available at https://doi.org/10.1091/mbc.e19-09-0500. Recommended citation: Lakdawala, M. F., Madhu, B., Faure, L., Vora, M., Padgett, R. W., & Gumienny, T. L. (2019). Genetic interactions between the DBL-1/BMP-like pathway anddpybody size–associated genes incaenorhabditis elegans. Molecular Biology of the Cell, 30(26), 3151–3160. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.en_US
dc.identifier.urihttps://doi.org/10.1091/mbc.E19-09-0500
dc.identifier.urihttps://hdl.handle.net/11274/12654
dc.language.isoen_USen_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.rights.licenseCC BY 4.0en_US
dc.subjectBone morphogenetic protein
dc.subjectNovel regulators
dc.subjectExtracellular matrix proteins
dc.titleGenetic interactions between the DBL-1/BMP-like pathway and dpy body size–associated genes in Caenorhabditis elegansen_US
dc.typeArticleen_US

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