Cloning and DNA sequence analysis of a plasmid from Helicobacter pylori
Helicobacter pylori is a pathogenic bacterium that resides in the gastric epithelium of some individuals and is widely regarded as a cause of type B gastritis and duodenal ulcer. Many bacterial species contain plasmid DNA which encodes virulence factors. Little is known about the plasmid DNAs found in some isolates of H. pylori. The purpose of this study was to clone a plasmid from Helicobacter pylori and to determine and analyze the DNA sequence.
A 3.5 kb plasmid (pHPM180) was isolated from a strain of H. pylori (HPM180) obtained from a patient with inactive duodenal ulcer. The plasmid was successfully cloned in both orientations in E. coli