Reverse phase high performance liquid chromatography with photodiode array detection method for the identification and detection of fat-soluble vitamins in plasma
| dc.contributor.advisor | Broughton, K. Shane | |
| dc.contributor.advisor | DiMarco, Nancy M. | |
| dc.contributor.advisor | Patterson, Mindy A. | |
| dc.contributor.author | Tucker, Sarah | |
| dc.date.accessioned | 2018-03-19T18:08:29Z | |
| dc.date.available | 2018-03-19T18:08:29Z | |
| dc.date.issued | 8/30/2017 | |
| dc.description.abstract | The purpose of this study was to establish a method for simultaneous evaluation and quantification of fat-soluble vitamins in human blood samples using reverse-phase high performance liquid chromatography coupled with photodiode array detection. The biochemical forms of fat-soluble vitamins analyzed include retinol, cholecalciferol, calcidiol, α-tocopherol, γ-tocopherol, and phylloquinone. This method is novel in the use of prostaglandin B1 as an internal standard for quantification and utilizes a simple method of extraction without derivatization or saponification. The method had a high degree of reproducibility as evidenced by coefficients of variation 1.7% and 2.5% of the mean for retinal and cholecalciferol respectively. | en_US |
| dc.identifier.uri | http://hdl.handle.net/11274/9367 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Organic chemistry | en_US |
| dc.subject | Nutrition | en_US |
| dc.subject | Pure sciences | en_US |
| dc.subject | Health and environmental sciences | en_US |
| dc.subject | Fat-soluble vitamins | en_US |
| dc.subject | High performance liquid chromatography | en_US |
| dc.subject | Hplc | en_US |
| dc.title | Reverse phase high performance liquid chromatography with photodiode array detection method for the identification and detection of fat-soluble vitamins in plasma | en_US |
| dc.type | Thesis | en_US |