Volume change associated with the low- to physiological-temperature conformational change in Thermomyces lanuginosus xylanase
| dc.contributor.advisor | Britt, Mark | |
| dc.contributor.author | Hollowell, Heather | |
| dc.contributor.committeeMember | Sheardy, Richard Dean | |
| dc.contributor.committeeMember | Rawashdeh-Omary, Manal | |
| dc.date.accessioned | 2018-08-07T17:33:24Z | |
| dc.date.available | 2018-08-07T17:33:24Z | |
| dc.date.issued | 2010-05 | |
| dc.description.abstract | Thermomyces lanuginosus is a thermophilic fungus with an optimal thriving temperature of 55°C. We have detected a conformational change in Thermomyces lanuginosus xylanase at 24°C and at pH = 7.0 and 1.0 atm pressure. This conformational change is revealed by an abrupt change in heat capacity as detected by slow-scan-rate differential scanning calorimetry and occurs at a temperature between that where crystals are formed for x-ray structure determination (4°C) and the physiological temperature (55°C). A volume change ΔV ≈-190 L/mol was calculated associated with the conversion from the low-temperature conformation to the higher-temperature conformation. The volume of the enzyme crystal structure, presumed to be the physiological structure, is 26 L/mole 14. We hypothesize this large, negative volume change indicates the low temperature form is either a molten globule or a random coil. | en_US |
| dc.identifier.uri | http://hdl.handle.net/11274/10213 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Pure sciences | en_US |
| dc.subject | Thermophilic fungi | en_US |
| dc.subject | Thermodynamics | en_US |
| dc.title | Volume change associated with the low- to physiological-temperature conformational change in Thermomyces lanuginosus xylanase | en_US |
| dc.type | Thesis | en_US |
| thesis.degree.college | College of Arts and Sciences | |
| thesis.degree.discipline | Chemistry | |
| thesis.degree.grantor | Texas Woman's University | en_US |
| thesis.degree.level | Master | en_US |
| thesis.degree.name | Master of Science | en_US |