Microarray analysis of estradiol-regulated genes in Arabidopsis thaliana seedlings

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dc.contributor.authorAdetunji, Kazeem Ademola
dc.contributor.committeeChairMaier, Camelia
dc.contributor.committeeMemberConrad-Webb, Heather
dc.contributor.committeeMemberDianna, Hynds
dc.date.accessioned2017-12-13T23:34:05Z
dc.date.available2017-12-13T23:34:05Z
dc.date.issued8/30/2012
dc.description.abstractSteroids are well-known regulators of physiological processes in plants and animals. The only known plant steroid hormones are brassinosteroids. Earlier studies demonstrated that animal steroid hormones have signaling activities in plants. The goal of this study was to determine the regulatory effects of estradiol on Arabidopsis gene expression. Seedlings were treated with 10 nM 17β-estradiol for three hours at the end of the seven-day cultures in liquid Murashige and Skoog medium. RNA was isolated from estradiol-treated and control plants and used for microarray analyses. From a total of 212 selected genes, 35% were up-regulated, 52% down-regulated and 13% non-responsive to estradiol treatment. Gene expression was altered mostly for secondary metabolism and CYP450 genes. Stress related genes were up-regulated indicating a possible synergistic effect of estradiol in plant growth and stress responses. Estradiol did not affect brassinosteroid biosynthesis genes. This study will help understand the steroid hormone and xenobionts effects on plants.en_US
dc.identifier.urihttp://hdl.handle.net/11274/8929
dc.language.isoen_USen_US
dc.publisherTexas Woman's Universityen_US
dc.subjectBiological sciencesen_US
dc.subjectBrassinosteroidsen_US
dc.subjectEstradiolen_US
dc.subjectGene expressionen_US
dc.subjectMicroarray analysesen_US
dc.subjectPlant steroid hormonesen_US
dc.subjectSeedlingsen_US
dc.titleMicroarray analysis of estradiol-regulated genes in Arabidopsis thaliana seedlingsen_US
dc.typeThesisen_US
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology
thesis.degree.disciplineBiology
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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