Innovations for Preservation of RNA for Studying Gene Expression

Simple item page
dc.contributor.authorEstrada, Kate
dc.contributor.authorGoyco, Maria
dc.contributor.authorBoling, Michaila G.
dc.contributor.authorTalapatra, Arpita
dc.contributor.authorMills, Nathaniel
dc.date.accessioned2021-03-19T17:44:26Z
dc.date.available2021-03-19T17:44:26Z
dc.date.issued2021
dc.descriptionCreative Arts and Research Symposium
dc.descriptionCreative Arts and Research Symposiumen_US
dc.description.abstractIn previous work, we used high salts to preserve RNA in tissues, but these solutions are toxic. We selected less toxic compounds to prepare solutions that preserve intact tissues, including their RNA and DNA, at high quality. We hypothesized that a solution containing a balanced salt, DMSO, and a polyanion can preserve intact RNA for later study and analysis of gene expression with the same quality as a high salt solution. Tissue samples were stored for ten months due to unplanned events (COVID-19) in solutions containing balanced salt, DMSO, and polyanions. After storage, the samples were extracted by homogenization of tissues in Trizol (the standard for extractions). Nucleic acid samples were analyzed for purity and yield. The integrity (size) of the RNA isolated was tested through agarose gel electrophoresis. We concluded that our solutions were successful in preserving RNA, but the Trizol extraction process was problematic when analyzing the results.
dc.description.departmentBiology
dc.description.sponsorshipTWU Center for Student Research and TWU Work Study Student Researchers Program
dc.identifier.urihttps://hdl.handle.net/11274/12820
dc.language.isoen_USen_US
dc.titleInnovations for Preservation of RNA for Studying Gene Expressionen_US
dc.typePosteren_US

Files

Original bundle

Now showing 1 - 2 of 2
No Thumbnail Available
Name:
0159_Estrada.mp4
Size:
9.49 MB
Format:
Description:
Presentation Video
Download
No Thumbnail Available
Name:
0159_Estrada.mp4.vtt
Size:
8.59 KB
Format:
Unknown data format
Description:
English subtitles
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.68 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Student Creative Arts and Research Symposium | 2021