Quantification of sugars and organic acids in 210 cucumber (cucurbis sativus) varieties for genome-wide association studies (GWAS)

There is limited information on the sugar and acid contents in Genome-Wide Association Study (GWAS) cucumbers. This study aimed to measure total soluble solid content (ºBrix), three main sugars via high-performance liquid chromatography (HPLC), pH, titratable acidity (TA), and three main organic acids via HPLC in 210 GWAS cucumber samples. A histogram and Agglomerative Hierarchical Clustering (AHC) were performed for each measured variable. The °Brix ranged from 1% – 4.6%. The content of fructose, glucose, and sucrose ranged from 2.88 - 62.28 mg/mL, 3.99 – 63.99 mg/mL, and 0.05 – 13.73 mg/mL, respectively. The pH ranged from 5.01 - 6.57. The TA ranged between 0.55 – 2.80 g/L. The content of malic, citric, and fumaric acid ranged from 0.08 – 2.64 mg/mL, 0.03 – 0.73 mg/mL, and 0.02 – 0.50 mg/mL, respectively. Results from this study can be beneficial for cucumber breeding program to produce a preferable tasting cucumber.