Student Creative Arts and Research Symposium
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Browsing Student Creative Arts and Research Symposium by Author "Campos, Dina"
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Item A mutational study of the energy transmission in E. coli ATP synthase(2019) Nguyen, Trang; Campos, Dina; Ikechukwu, Ifeoma; Valdez, Neydy; Hernandez-Alvarado, Jose; Li, YunxiangATP synthase catalyzes ATP synthesis by oxidative phosphorylation. With the unique mechanism and structure of ATP synthase, the energy transmission between the rotor and stator complex plays a vital role to maintain its proper function. Our previous study has discussed that upon γC87K mutation, a stronger interaction with βE381 could refrain the rotor complex from smooth spin, leading to insufficient energy coupling. In this study, we engineered alanine mutations to these residues to mimic a weaker rotor/stator interaction, and we found that multiple alanine mutation also impairs the enzyme performance. This research will add more pieces to understand the energy flow in ATP synthase.