Exposure to alcohol interrupts adipose cell maturation, attenuates adiponectin expression, and contributes to inflammatory markers in 3T3-F442 pre-adipocytes

Simple item page
dc.contributor.advisorDavis, Kathleen Elizabeth
dc.creatorShafiei, Mahnoush Sophia
dc.date.accessioned2019-02-13T17:26:05Z
dc.date.created2018-12
dc.date.issued12/20/2018
dc.date.submittedDec-18
dc.date.updated2019-02-13T17:26:05Z
dc.description.abstractDrinking alcohol during pregnancy interrupts cellular development, which may have far-reaching health effects on the fetus in its embryonic state and later as a young child. Adipose tissue (AT) is an important target for alcohol action. Alcohol disrupts the synthesis of a wide variety of peptides and adipokines, as well as the endocrine activity of adipose tissue. Adiponectin is an anti-inflammatory and insulin-sensitizing adipokine that is involved in fatty acid breakdown; it is produced in adipose tissue. In this project, it is hypothesized that alcohol disrupts adipose cell development and reduces adiponectin (ApN) expression, concomitant with an elevation of pro-inflammatory cytokines and reduction in anti-inflammatory cytokines. The data suggest that alcohol exposure in 3T3-F442 pre-adipocytes reduces adipocyte proliferation. Pre-adipocytes were exposed to 0.0% (control), 0.25%, 0.5%, 1%, 1.5%, 2%, and 2.5% alcohol solution for 48 hours. Triglycerides and the expression of ApN and several pro- and anti-inflammatory cytokines were measured. Nile Red (NR) staining was used for the detection of adipogenesis and adipocyte differentiation. The results show triglyceride reduction in 3T3-F442 adipocytes and a significant reduction in adipocyte differentiation in comparison with control (non-alcohol-treated) cells. Furthermore, ApN secretion was reduced in 3T3-F442 cells in response to alcohol. The pro-inflammatory cytokines, IL-6, IL-13, IL-1b, TNF-α, and INF-γ were increased, whereas the anti-inflammatory cytokines, including IL-4 and IL-10, IL-12, were reduced. In conclusion, exposure of alcohol reduced differentiation of 3T3-F442 pre-adipocytes to adipocytes. It was demonstrated in this project that alcohol impairs ApN secretion and increases pro-inflammatory cytokines. The results help to establish the potential role of alcohol in promoting inflammation and reducing adiponectin expression in developing pre-adipocytes, suggesting alcohol may be disruptive in metabolism by disruption of adipose cell differentiation.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/11274/11024
dc.language.isoen
dc.subjectAdiponectin
dc.subjectInterferon
dc.titleExposure to alcohol interrupts adipose cell maturation, attenuates adiponectin expression, and contributes to inflammatory markers in 3T3-F442 pre-adipocytes
dc.typeThesis
dc.type.materialtext
local.embargo.lift12/1/2020
local.embargo.terms12/1/2020
thesis.degree.departmentNutrition and Food Sciences
thesis.degree.disciplineNutrition
thesis.degree.grantorTexas Woman's University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
SHAFIEI-DISSERTATION-2018.pdf
Size:
2.35 MB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.85 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
1.85 KB
Format:
Plain Text
Description:
Download

Collections

Nutrition & Food Sciences
2018 Theses and Dissertations