Myco-synthesis of silver nanoparticles and their bioactive role against pathogenic microbes

Date
2023-04
Authors
Abdel-Hadi, Ahmed
Iqbal, Danish
Alharbi, Raed
Jahan, Sadaf
Darwish, Omar
Alshehri, Bader
Banawas, Saeed
Palanisamy, Manikanadan
Ismail, Ahmed
Aldosari, Sahar
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract

Simple Summary: The relatively high prevalence of microbial infections and the rising resistance to traditional antibiotics are the causes of the need for revolutionary antibiotics. Nanotechnology, a technique that employs materials featuring nanometer size, has grown in popularity for therapeutic uses and is very intriguing as a means of eradicating or limiting the activity of several pathogens. Silver nanoparticles have been shown to have antimicrobial properties against fungi and bacteria. The unique properties of silver nanoparticles, such as their high surface-area-to-volume ratio and the ability to release silver ions, can cause damage to the microbial cell membrane, interfere with cellular processes, and make them effective against a wide range of microorganisms. Synthesis of nanoparticles via natural products could potentiate their therapeutic activities. Moreover, phosphatase enzyme is also known to possess antimicrobial effects, and there is a fungus (Fusarium oxysporum) reported to have phosphatase enzymes in its extracellular fluid. Therefore, we focused on synthesizing silver nanoparticles by using extracellular proteins released by Fusarium oxysporum and thereafter evaluated its biological activities against pathogenic microbes. Our findings illustrated that synthesized nanoparticles showed prominent anti-microbicidal activities against various pathogenic bacterial and fungal species. Thus, these nanoparticles may be used against drug-resistant infections.

Abstract: Nanotechnology based on nanoscale materials is rapidly being used in clinical settings, particularly as a new approach for infectious illnesses. Recently, many physical/chemical approaches utilized to produce nanoparticles are expensive and highly unsafe to biological species and ecosystems. This study demonstrated an environmentally friendly mode of producing nanoparticles (NPs) where Fusarium oxysporum has been employed for generation of silver nanoparticles (AgNPs), which were further tested for their antimicrobial potentials against a variety of pathogenic microorganisms. The characterization of NPs was completed by UV–Vis spectroscopy, DLS and TEM, where it has been found that the NPs were mostly globular, with the size range of 50 to 100 nm. The myco-synthesized AgNPs showed prominent antibacterial potency observed as zone of inhibition of 2.6 mm, 1.8 mm, 1.5 mm, and 1.8 mm against Vibrio cholerae, Streptococcus pneumoniae, Klebsiella pneumoniae and Bacillus anthracis, respectively, at 100 µM. Similarly, at 200 µM for A. alternata, A. flavus and Trichoderma have shown zone of inhibition as 2.6 mm, 2.4 mm, and 2.1 mm, respectively. Moreover, SEM analysis of A. alternata confirmed the hyphal damage where the layers of membranes were torn off, and further EDX data analysis showed the presence of silver NPs, which might be responsible for hyphal damage. The potency of NPs may be related with the capping of fungal proteins that are produced extracellularly. Thus, these AgNPs may be used against pathogenic microbes and play a beneficial role against multi-drug resistance.

Description
Article originally published by Biology, 12(5), 661. English. Published April 2023. https://doi.org/10.3390/biology12050661
Keywords
Biomedical, Drug resistance, Fungal extract, Microbicidal, Nanotechnology, Silver nanoparticle
Citation
This is the published version of an article that is available at https://doi.org/10.3390/biology12050661. Recommended citation: Abdel-Hadi, A., Iqbal, D., Alharbi, R., Jahan, S., Darwish, O., Alshehri, B., Banawas, S., Palanisamy, M., Ismail, A., Aldosari, S., Alsaweed, M., Madkhali, Y., Kamal, M., & Fatima, F. (2023). Myco-synthesis of silver nanoparticles and their bioactive role against pathogenic microbes. Biology, 12(5), 661. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.