Identification of mammalian mediator subunits with similarities to yeast mediator subunits srb5, srb6, med11, and ROX3

Simple item page
dc.contributor.authorSato, Shigeo
dc.contributor.authorTomomori-Sato, Chieri
dc.contributor.authorBanks, Charles A.S.
dc.contributor.authorSorokina, Irina
dc.contributor.authorParmely, Tari J.
dc.contributor.authorKong, Stephanie E.
dc.contributor.authorJin, Jingji
dc.contributor.authorCai, Yong
dc.contributor.authorLane, William S.
dc.contributor.authorBrower, Christopher S.
dc.contributor.authorConaway, Ronald C.
dc.contributor.authorConaway, Joan Weliky
dc.creator.orcidhttps://orcid.org/0000-0003-0289-5541
dc.date.accessioned2023-01-27T17:32:44Z
dc.date.available2023-01-27T17:32:44Z
dc.date.issued2003
dc.description.abstractThe Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator subunit Med8 and partially purified a Med8-containing Mediator complex from rat liver nuclei (Brower, C. S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R. D., Malik, S., Lane, W. S., Sorokina, I., Roeder, R. G., Conaway, J. W., and Conaway, R. C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353–10358). Analysis of proteins present in the most highly purified Med8-containing fractions by tandem mass spectrometry led to the identification of many known mammalian Mediator subunits, as well as four potential Mediator subunits exhibiting sequence similarity to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Here we present direct biochemical evidence that these four proteins are bona fide mammalian Mediator subunits. In addition, we identify direct pairwise binding partners of these proteins among the known mammalian Mediator subunits. Taken together, our findings identify a collection of novel mammalian Mediator subunits and shed new light on the underlying architecture of the mammalian Mediator complex.en_US
dc.identifier.citationThis is the published version of an article that is available at https://doi.org/10.1074/jbc.c300054200. Recommended citation:Sato, S., Tomomori-Sato, C., Banks, C. A. S., Sorokina, I., Parmely, T. J., Kong, S. E., Jin, J., Cai, Y., Lane, W. S., Brower, C. S., Conaway, R. C., & Conaway, J. W. (2003). Identification of mammalian mediator subunits with similarities to yeast mediator subunits srb5, srb6, med11, and ROX3. Journal of Biological Chemistry, 278(17), 15123–15127. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.en_US
dc.identifier.urihttps://hdl.handle.net/11274/14345
dc.identifier.urihttps://doi.org/10.1074/jbc.c300054200
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.rights.licenseCC-BY 4.0
dc.subjectThyroid hormone receptor-associated proteinen_US
dc.subjectSrb-Med-containing cofactoren_US
dc.subjectVitamin D receptor-interacting proteinen_US
dc.titleIdentification of mammalian mediator subunits with similarities to yeast mediator subunits srb5, srb6, med11, and ROX3en_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Brower-Identification of Mammalian Mediator Subunits.pdf
Size:
275.13 KB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.68 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Biology