Regulation of Expression of the M143 Promoter of Mouse Cytomegalovirus

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dc.contributor.authorEluru, Seneetha Reddyen_US
dc.contributor.committeeChairHanson, Laura K.
dc.contributor.committeeMemberConrad-Webb, Heather
dc.contributor.committeeMemberMills, Nathaniel C.
dc.date.accessioned2014-04-04T19:00:57Z
dc.date.available2014-04-04T19:00:57Z
dc.date.issued1/1/2013en_US
dc.description.abstractThe objective of this research is to examine the regulation of an essential gene (m143) in MCMV that has sequence and functional homology to HCMV genes IRS1/TRS1. All of these prevent the shutdown of protein synthesis that is an antiviral defense. Little is known about the regulation of these genes at either the transcriptional or posttranscriptional levels. Sequential deletion mutant analysis of the m143 promoter was done in the context of an SEAP reporter plasmid. The results indicated that viral proteins are required for activation and the presence of repressor-binding sites and activation-binding sites. EMSA shows that SP1 may be important for the activation. Two important viral transcriptional regulators IE1 and IE3, each alone weakly activates the m143 promoter, but together synergize to efficiently activate this promoter. The absence of repression with co-transfection experiments support repression by other viral proteins. Such studies may lead to novel treatments for cytomegalovirus infection.en_US
dc.identifier.urihttp://hdl.handle.net/11274/346
dc.language.isoen_USen_US
dc.subjectVirologyen_US
dc.subjectMicrobiologyen_US
dc.subjectBiologyen_US
dc.titleRegulation of Expression of the M143 Promoter of Mouse Cytomegalovirusen_US
dc.typeThesisen_US
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology
thesis.degree.disciplineBiology
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMaster
thesis.degree.nameMaster of Science

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