Feedback regulation of BMP signaling by Caenorhabditis elegans cuticle collagens

Date

2020-03-31

Authors

Gumienny, Tina L.
Madaan, Uday
Faure, Lionel
Chowdhury, Albar
Ahmed, Shahrear
Ciccarelli, Emma J.
Savage-Dunn, Cathy

Journal Title

Journal ISSN

Volume Title

Publisher

American Society for Cell Biology

Abstract

Cellular responsiveness to environment, including changes in extracellular matrix (ECM), is critical for normal processes such as development and wound healing, but can go awry, as in oncogenesis and fibrosis. One type of molecular pathway contributing to this responsiveness is the BMP signaling pathway. Owing to their broad and potent functions, BMPs and their pathways are regulated at multiple levels. In Caenorhabditis elegans, the BMP ligand DBL-1 is a regulator of body size. We previously showed that DBL-1/BMP signaling determines body size through transcriptional regulation of cuticle collagen genes. We now identify feedback regulation of DBL-1/BMP through analysis of four DBL-1–regulated collagen genes. Inactivation of any of these genes reduces DBL-1/BMP signaling, measured by a pathway activity reporter. Furthermore, depletion of these collagens reduces GFP::DBL-1 fluorescence and acts unexpectedly at the level of dbl-1 transcription. We conclude that cuticle, a specialized ECM, impinges on DBL-1/BMP expression and signaling. Interestingly, the feedback regulation of DBL-1/BMP signaling by collagens is likely to be contact independent due to physical separation of the cuticle from DBL-1–expressing cells in the ventral nerve cord. Our results provide an entry point into a novel regulatory mechanism for BMP signaling, with broader implications for mechanical regulation of gene expression.

Description

Article originally published in Molecular Biology of the Cell, 31(8), 825–832. English. Published online 2020. https://doi.org/10.1091/mbc.e19-07-0390

Keywords

Extracellular matrix, Novel regulatory mechanism, Mechanical regulation of gene expression

Citation

This is the published version of an article that is available at https://doi.org/10.1091/mbc.e19-07-0390. Recommended citation: Madaan, U., Faure, L., Chowdhury, A., Ahmed, S., Ciccarelli, E. J., Gumienny, T. L., & Savage-Dunn, C. (2020). Feedback regulation of BMP signaling by Caenorhabditis elegans cuticle collagens. Molecular Biology of the Cell, 31(8), 825–832. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.

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