Using Live Imaging of Cells to Optimize Experimental Conditions

Date
2021
Authors
Froman, Cheyanne
Navarro Gutierrez, Fatima D.
Spencer, Juliet V.
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Abstract

Many human diseases can be investigated in the laboratory using in vitro cell culture systems. However, these experiments require optimal culture conditions in order to serve as useful models. Human cytomegalovirus (HCMV) is a widespread pathogen and the leading infectious cause of birth defects. We plan to investigate the impact of chemokine antagonists on HCMV replication. To initiate these studies, we used the Incucyte Live Cell Analysis system to determine optimal culture conditions for the growth of adult retinal pigment epithelial 19 (ARPE-19) cells. We seeded a range of cell densities and captured phase-contrast images of the cultures at four hour intervals for 96 hours. The images were analyzed by applying a mask to identify and count cells in each field, and the results indicated an optimal seeding density for future experiments aimed at blocking HCMV replication in epithelial cells.

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Creative Arts and Research Symposium
Creative Arts and Research Symposium
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