Leukocyte subset changes in response to a 164-km road cycle ride in a hot environment

Date

2016

Authors

Duplanty, Anthony A.
Luk, Hui Ying
McKenzie, Amy L.
Budnar, Ronald G.
Levitt, Danielle
Fernandez, Alex
Lee, Elaine C.
Armstrong, Lawrence E.
Vingren, Jakob L.

Journal Title

Journal ISSN

Volume Title

Publisher

Western Kentucky University

Abstract

The purpose of this observational study was to determine the circulating leukocyte subset response to completing the 2013 Hotter’N Hell Hundred recreational 164-km road cycle event in a hot and humid environmental condition. Twenty-eight men and four women were included in this study. Whole blood samples were obtained 1-2 hours before (PRE) and immediately after (POST) the event. Electronic sizing/sorting and cytometry were used to determine complete blood counts (CBC) including neutrophil, monocyte, and lymphocyte subsets. The concentration of circulating total leukocytes (103·µL-1) increased 134% from PRE to POST with the greatest increase in neutrophils (319%, p<0.0001). Circulating monocytes (including macrophages) increased 24% (p=0.004) and circulating lymphocytes including B and T cells increased 53% (p<0.0001). No association was observed between rolling time or relative intensity and leukocyte subset. Completing the Hotter n’ Hell Hundred (HHH), a 100 mile recreational cycling race in extreme (hot and humid) environmental conditions, induces a substantial increase in total leukocytes in circulation. The contribution of increases in specific immune cell subsets is not equal, with neutrophils increasing to greater than 4-fold starting values from PRE to POST race. It is likely that exercise in stressful environmental conditions affects the complement of circulating immune cells, although activational state and characterization of specific leukocyte subsets remains unclear. The observed increase in circulating cell sub-populations suggests that the circulating immune surveillance system may be acutely affected by exercise in hot and humid conditions. **This article was originally published by the International Journal of Exercise Science: Luk, Hui Ying; Mckenzie, Amy L.; Duplanty, Anthony A.; Budnar, Ronald G.; Levitt, Danielle; Fernandez, Alex; Lee, Elaine C.; Armstrong, Lawrence E.; and Vingren, Jakob L. (2016) "Leukocyte Subset Changes in Response to a 164-km Road Cycle Ride in a Hot Environment," International Journal of Exercise Science:Vol.9:Iss.1. Available at https://digitalcommons.wku.edu/ijes/vol9/iss1/5.

Description

Article originally published in International Journal of Exercise Science, 9(1), 34–46. English. Published online 2016. https://digitalcommons.wku.edu/ijes/vol9/iss1/5/

Keywords

White blood cells, Heat, Ultra-endurance, Neutrophils, Monocyte, Exercise, Lymphocyte

Citation

This is the published version of an article that is available at https://digitalcommons.wku.edu/ijes/vol9/iss1/5. Recommended citation: Luk, H.Y; Mckenzie, A.L.; Duplanty, A.A.; Budnar, R. G.; Levitt, D.; Fernandez, A.; Lee, E.C.; Armstrong, L.E.; and Vingren, J.L. (2016) Leukocyte subset changes in response to a 164-km road cycle ride in a hot environment. International Journal of Exercise Science, 9(1), 34-46. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.

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