Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus

Date

1993-05

Authors

Sparks, Mary Jean

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Abstract

Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3\sp end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data.

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Keywords

Bean Commen Mosaic Virus, Nucleotide sequence

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