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dc.contributor.authorMcKim, Mikeal
dc.date.accessioned2018-04-23T20:43:44Z
dc.date.available2018-04-23T20:43:44Z
dc.date.issued2015-08-30
dc.identifier.urihttp://hdl.handle.net/11274/9637
dc.description.abstractTelomeres are protein DNA complexes found at the ends of eukaryotic chromosomes. The human telomere sequence has a G-rich strand, (TTAGGG), complemented to a C-rich strand, (CCCTAA). Previous studies of the G-rich strand indicate that this sequence folds into a distinct conformation, designated as a G quadruplex. The C-rich strand has also been shown to form an unusual structure, under slightly acidic conditions, designated as a DNA i-motif. We are investigating the role of the loops on the structure and stability of the conformations formed from (CCCXXX)4, where X is all permutations of A and/or T. To understand the significance of the presence of the loops, structure and stability will be studied for the dimer and tetramer conformations formed from (CCCTAA)x, where X is either a 1 or a 2. These results indicate that the sequence context and the presence or absence of the loops, influences both the midpoint of the heat induced i-motif to single strand transition (Tm) at pH = 5.0, as well as, the midpoint of the H + induced single strand to i-motif transition (pHmp) at 25 °C.
dc.language.isoen_USen_US
dc.subjectPure sciences
dc.subjectBiological sciences
dc.subjectCytosine
dc.subjectDNA
dc.subjectEnthalpy
dc.subjectEntropy
dc.subjectI-motif
dc.subjectProtonation
dc.titleStructural and thermodynamic studies of a DNA i-motif: Investigating the monomer, dimer, and tetramer human telomere sequence contexten_US
dc.typeThesisen_US
thesis.degree.departmentChemistry and Biochemistry
thesis.degree.disciplineChemistry
thesis.degree.grantorTexas Woman's University
thesis.degree.levelMaster
thesis.degree.nameMaster of Science
dc.contributor.committeeChairSheardy, Richard Dean
dc.contributor.committeeMemberAnderson, Mary
dc.contributor.committeeMemberBritt, Mark
thesis.degree.collegeCollege of Arts and Sciences


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