Chemistry & Biochemistry - Faculty & Staff
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The nucleotide binding affinities of two critical conformations of escherichia coli ATP synthase
(Elsevier, 2021)ATP synthase is essential in aerobic energy metabolism, and the rotary catalytic mechanism is one of the core concepts to understand the energetic functions of ATP synthase. Disulfide bonds formed by oxidizing a pair of ... -
Interaction between ΓC87 and ΓR242 residues participates in energy coupling between catalysis and proton translocation in escherichia coli ATP synthase
(Elsevier, 2019)Functioning as a nanomotor, ATP synthase plays a vital role in the cellular energy metabolism. Interactions at the rotor and stator interface are critical to the energy transmission in ATP synthase. From mutational studies, ... -
Identification of two segments of the γ subunit of ATP synthase responsible for the different affinities of the catalytic nucleotide-binding sites
(American Society for Biochemistry and Molecular Biology, 2019)ATP synthase uses a rotary mechanism to couple transmembrane proton translocation to ATP synthesis and hydrolysis, which occur at the catalytic sites in the β subunits. In the presence of Mg2+, the three catalytic sites ... -
Site-specific pegylation crosslinking of L-asparaginase subunits to improve its therapeutic efficiency
(Cold Spring Harbor Laboratory, 2018)L-Asparaginase is an enzyme successfully being used in the treatment of acute lymphoblastic leukemia, acute myeloid leukemia, and non-Hodgkin’s lymphoma. However, some disadvantages still limit its full application potential, ... -
Correction: Machine-learning-assisted screening of pure-silica zeolites for effective removal of linear siloxanes and derivatives
(Royal Society of Chemistry, 2020)As emerging organic contaminants, siloxanes have severe impacts on the environment and human health. Simple linear siloxanes and derivates, trimethylsilanol (TMS), dimethylsilanediol (DMSD), monomethylsilanetriol (MMST), ... -
A wireless resonant LC sensor for glucose detection
(MDPI, 2022)This paper proposes a wireless resonant inductive and capacitive (LC) sensor for glucose sensing. A sensor composed of a capacitor with interdigital electrodes and an inductor for glucose sensing is presented. Resonance ... -
Identification of mammalian mediator subunits with similarities to yeast mediator subunits srb5, srb6, med11, and ROX3
(Elsevier, 2003)The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator subunit Med8 and partially ... -
The elongin B ubiquitin homology domain
(Elsevier, 1999)Mammalian Elongin B is a 118-amino acid protein composed of an 84-amino acid amino-terminal ubiquitin-like domain and a 34-amino acid carboxyl-terminal tail. Elongin B is found in cells as a subunit of the heterodimeric ... -
A molecular basis for stabilization of the von hippel-lindau (VHL) tumor suppressor protein by components of the VHL ubiquitin ligase
(Elsevier, 2002)The multiprotein von Hippel-Lindau (VHL) tumor suppressor (CBCVHL, Cul2-ElonginBC-VHL) and SCF (Skp1-Cul1/Cdc53-F-box protein) complexes are members of structurally related families of E3 ubiquitin ligases that use a ... -
A mammalian homolog of drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator Complex
(Elsevier, 2003)The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors. We previously partially purified a Med8-containing ... -
A mammalian mediator subunit that shares properties with saccharomyces cerevisiae mediator subunit CSE2
(Elsevier, 2004)The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. We previously identified and partially purified a mammalian Mediator complex ... -
Arginyltransferase, its specificity, putative substrates, bidirectional promoter, and splicing-derived isoforms
(Elsevier, 2006)Substrates of the N-end rule pathway include proteins with destabilizing N-terminal residues. Three of them, Asp, Glu, and (oxidized) Cys, function through their conjugation to Arg, one of destabilizing N-terminal ... -
Ablation of arginylation in the mouse N-end rule pathway: Loss of fat, higher metabolic rate, damaged spermatogenesis, and neurological perturbations
(Public Library of Science, 2009)In the N-end rule pathway of protein degradation, the destabilizing activity of N-terminal Asp, Glu or (oxidized) Cys residues requires their conjugation to Arg, which is recognized directly by pathway’s ubiquitin ligases. ... -
Mouse DFA is a repressor of TATA-box promoters and interacts with the Abt1 activator of basal transcription
(Elsevier, 2010)Our study of the mouse Ate1 arginyltransferase, a component of the N-end rule pathway, has shown that Ate1 pre-mRNA is produced from a bidirectional promoter that also expresses, in the opposite direction, a previously ... -
Degradation of the separase-cleaved REC8, a meiotic cohesin subunit, by the N-end rule pathway
(Elsevier, 2016)The Ate1 arginyltransferase (R-transferase) is a component of the N-end rule pathway, which recognizes proteins containing N-terminal degradation signals called N-degrons, polyubiquitylates these proteins, and thereby ... -
Analyzing N-terminal arginylation through the use of peptide arrays and degradation assays
(Elsevier, 2016)Nα-terminal arginylation (Nt-arginylation) of proteins is mediated by the Ate1 arginyltransferase (R-transferase), a component of the Arg/N-end rule pathway. This proteolytic system recognizes proteins containing N-terminal ... -
The N termini of tar DNA-binding protein 43 (TDP43) C-terminal fragments influence degradation, aggregation propensity, and morphology
(Taylor and Francis, 2018)Fragments of the TAR DNA-binding protein 43 (TDP43) are major components of intracellular aggregates associated with amyotrophic lateral sclerosis and frontotemporal dementia. A variety of C-terminal fragments (CTFs) ... -
Beta-amyloid induces apoptosis of neuronal cells by inhibition of the Arg/N-end rule pathway proteolytic activit
(Impact Journals, 2019)Alzheimer’s disease (AD) is accompanied by the dysfunction of intracellular protein homeostasis systems, in particular the ubiquitin-proteasome system (UPS). Beta-amyloid peptide (Aβ), which is involved in the processes ... -
BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43
(Elsevier, 2022)Neurodegeneration is associated with the aggregation of proteins bearing solvent-exposed hydrophobicity as a result of their misfolding and/or proteolytic cleavage. An understanding of the cellular protein quality control ... -
Genetic mutations in the S-loop of human glutathione synthetase: Links between substrate binding, active site structure and allostery
(Elsevier, 2018)The second step in the biosynthesis of the cellular antioxidant glutathione (GSH) is catalyzed by human glutathione synthetase (hGS), a negatively cooperative homodimer. Patients with mutations in hGS have been reported ...
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