Effect of Delta-Tocotrienol and Grape Seed Polyphenol on Lipid Profile in C57BL/6J Mice with a Western-Like Diet-Included Non-Alcoholic Steatohepatitis

Abstract

Non-alcoholic steatohepatitis (NASH), the second phase in liver disease, occurs when adults consume high calorie, high fat diets. Fat accumulated in the liver causes inflammation and irreversible hardening. Grape seed polyphenol (GSP) and d-tocotrienol (d-T3) may have benefits in reducing NASH. We observed the effects of dietary GSP & d-T3 (separated and combined: GSP - 2%; d-T3 - 0.05%) in C57BL/6J mice fed a high calorie high fat diet for 20 weeks. Hepatic cholesterol, triglycerides, and vitamin E; serum cholesterol, triglycerides, vitamin E, and free fatty acids were assayed. Hepatic total cholesterol was lowest in GSP diet and GSP(2%)+dT3(0.05%) diet groups. Hepatic triglycerides were lowest in the GSP(2%)+dT3(0.05%) diet group. Hepatic vitamin E levels were lowest in the GSP(2%)+dT3(0.05%) diet group. Serum cholesterol was lowest in GSP diet and GSP(2%)+dT3(0.05%) diet groups. Serum vitamin E was lowest in the GSP diet group. Serum triglycerides and FFA's were similar among the groups.