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dc.contributor.authorSamara, Hala
dc.contributor.authorPislariu, Catalina
dc.date.accessioned2022-05-12T22:14:20Z
dc.date.available2022-05-12T22:14:20Z
dc.date.issued2022
dc.identifier.urihttps://hdl.handle.net/11274/13706
dc.description.abstractLeguminous plants can grow without nitrogenous fertilizer because they acquire ammonia through symbiosis with nitrogen (N2)-fixing bacteria (rhizobia) from the soil. In symbiotic Nitrogen Fixation (SNF), new organs develop (root nodules) providing a niche for rhizobia to fix N2. The Medicago truncatula-Sinorhizobium meliloti association is a useful genetic model to study SNF. Earlier, a nodule- specific Medicago gene (MtNPD1) encoding a PLAT domain protein, was reported. Mutants with disrupted MtNPD1 develop small nodules where rhizobia don’t fix N2 and are quickly degraded. The PLAT domain is predicted to be involved in protein-protein and protein-lipid interaction. Extensive membrane degradation is common in npd1 mutants; therefore, the goal of this work is to determine if MtNPD1 interacts with membrane lipids to ensure membrane stability. MtNPD1 recombinant proteins are being generated and the purified proteins will be used to run Protein-Lipid Overlay (PLO) assay. Representative results will be presented. (Faculty Sponsor: Dr. Catalina Pislariu)en_US
dc.language.isoen_USen_US
dc.titleExploring lipid binding capacity of the Medicago truncatula nodule-specific PLAT domain 1 (MtNPD1) proteinen_US
dc.typePosteren_US


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