|dc.description.abstract||TBIs stands for traumatic brain injury which causes permanent loss
of neural tissue. This experiment focused on pressure caused TBI
and its resulting pathology. Cerebral organoids are in-vitro 3-
dimensional stem cell cultures that display cerebral cortical regions
similar to a developing human brain. Cerebral organoids were used
because they potentially offer a more humanistic model of
organization. The cerebral organoids were grown using the Pasca
protocol and then to model a pressure induced TBI, the organoids
are loaded into a tabletop blast chamber. The sections were stained
using immunohistochemistry for activated caspase 3, which is an
apoptosis marker to determine if the organoids reacted the same
as a human brain would. In the cerebral organoids the frequency of
the blast and the amount of cell death have a positive correlation.
Thus, the dose response to different frequencies gives a threshold
of cell death like that of a human blast TBI. (Faculty Sponsor: Dr.