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dc.contributor.authorRodriguez, Erica
dc.date.accessioned2020-09-18T19:37:19Z
dc.date.available2020-09-18T19:37:19Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/11274/12495
dc.descriptionCreative Arts and Research Symposiumen_US
dc.description.abstractUnderstanding anatomical circuitries promotes the development of novel therapeutics to manage neurological pathologies. Tract tracing is a neuroanatomical approach that allows visualization of neural pathways. Wheat germ agglutinin (WGA) is a transsynaptic tracer taken up by neurons and passed to other networked neurons. WGA can be conjugated to a fluorescent dye (WGA-488) to be observed with a microscope. The goal of our study was to develop methodology using WGA-488 to visualize connectivity of the sensory nerves of the face into brainstem nuclei. We injected various volumes of 1% WGA-488 into the rat cheek or the jaw joint. Post-mortem sensory ganglia and brainstem were collected 3- or 7-days later, sectioned, and analyzed with a microscope. We found that 50 μL WGA-488 was observed in the trigeminal ganglia and several brainstem nuclei at 3-days post-tracer injection. This methodology can now be used to characterize the functional neurochemistry of the orofacial sensory neurons.
dc.description.sponsorshipDayna Averitten_US
dc.language.isoen_USen_US
dc.titleNeuronal Tract Tracing in the Rat Trigeminal Sensory Systemen_US
dc.typePresentationen_US


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