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dc.contributor.authorBasu, Paramita
dc.contributor.authorTongkhuya, Sirima A.
dc.contributor.authorHarris, Taylor L.
dc.contributor.authorRiley, Angela R.
dc.contributor.authorMaier, Camelia
dc.contributor.authorGranger, John
dc.contributor.authorWojtaszek, Jennie
dc.contributor.authorAveritt, Dayna L.
dc.date.accessioned2019-09-12T20:44:36Z
dc.date.available2019-09-12T20:44:36Z
dc.date.issued2019-09-03
dc.identifier.citationThis is a published version of an article that is available at: https://doi.org/10.3389/fphar.2019.00958. Recommended citation: Basu, P., Tongkhuya, S. A., Harris, T. L., Riley, A. R., Maier, C., Granger, J., Wojtaszek, J., & Averitt, D. L. (2019). Euphorbia bicolor (Euphorbiaceae) latex phytochemicals induce long-lasting non-opioid peripheral analgesia in a rat model of inflammatory pain. Frontiers in Pharmacology, 10. https://doi.org/10.3389/fphar.2019.00958. This item has been deposited in accordance with publisher copyright and licensing terms and with the author’s permission.en_US
dc.identifier.urihttps://hdl.handle.net/11274/11806
dc.identifier.urihttps://doi.org/10.3389/fphar.2019.00958
dc.descriptionArticle originally published in the Frontiers in Pharmacology, 10. English. Published online September 2019. https://doi.org/10.3389/fphar.2019.00958
dc.description.abstractThe negative side effects of opioid-based narcotics underscore the search for alternative non-opioid bioactive compounds that act on the peripheral nervous system to avoid central nervous system-mediated side effects. The transient receptor potential V1 ion channel (TRPV1) is a peripheral pain generator activated and sensitized by heat, capsaicin, and a variety of endogenous ligands. TRPV1 contributes to peripheral sensitization and hyperalgesia, in part, via triggering the release of proinflammatory peptides, such as calcitonin gene-related peptide (CGRP), both locally and at the dorsal horn of the spinal cord. Ultrapotent exogenous TRPV1 agonists, such as resiniferatoxin identified in the latex of the exotic Euphorbia resinifera, trigger hyperalgesia followed by long lasting, peripheral analgesia. The present study reports on the analgesic properties of Euphorbia bicolor, a relative of E. resinifera, native to the Southern United States. The study hypothesized that E. bicolor latex extract induces long-lasting, non-opioid peripheral analgesia in a rat model of inflammatory pain. Both inflamed and non-inflamed adult male and female rats were injected with the methanolic extract of E. bicolor latex into the hindpaw and changes in pain behaviors were reassessed at various time points up to 4 weeks. Primary sensory neuron cultures also were treated with the latex extract or vehicle for 15 min followed by stimulation with the TRPV1 agonist capsaicin. Results showed that E. bicolor latex extract evoked significant pain behaviors in both male and female rats at 20 min post-injection and lasting around 1–2 h. At 6 h post-injection, analgesia was observed in male rats that lasted up to 4 weeks, whereas in females the onset of analgesia was delayed to 72 h post-injection. In sensory neurons, latex extract significantly reduced capsaicin-evoked CGRP release. Blocking TRPV1, but not opioid receptors, attenuated the onset of analgesia and capsaicin-induced CGRP release. Latex was analyzed by mass spectrometry and eleven candidate compounds were identified and reported here. These findings indicate that phytochemicals in the E. bicolor latex induce hyperalgesia followed by peripheral, non-opioid analgesia in both male and female rats, which occurs in part via TRPV1 and may provide novel, non-opioid peripheral analgesics that warrant further examination.en_US
dc.description.sponsorshipThis research was supported by Texas Woman’s University (TWU) Research Enhancement Grants awarded to CM and DA, a TWU Chancellor’s Research Fellowship awarded to DA, TWU Quality Enhancement Program Learn by Doing grants awarded to PB and ST, and a TWU Center for Student Research Small Grant awarded to DLA and PB.en_US
dc.language.isoen_USen_US
dc.publisherFrontiers in Pharmacology
dc.subjectAnalgesiaen_US
dc.subjectDiterpenesen_US
dc.subjectEuphorbiaen_US
dc.subjectFlavonoidsen_US
dc.subjectHyperalgesiaen_US
dc.subjectInflammationen_US
dc.subjectTRPV1en_US
dc.subjectUPLC-ESI-MS/MSen_US
dc.titleEuphorbia bicolor (Euphorbiaceae) latex phytochemicals induce long-lasting non-opioid peripheral analgesia in a rat model of inflammatory painen_US
dc.typeArticleen_US
dc.rights.licenseCopyright © 2019 Basu, Tongkhuya, Harris, Riley, Maier, Granger, Wojtaszek and Averitt. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.creator.orcidhttps://orcid.org/0000-0001-8345-4988


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